Purification & Size Determination of Green & Blue Fluorescent Proteins

255 web

Purification & Size Determination of Green & Blue Fluorescent Proteins

£95.00 Ex VAT

For 6 student groups

SKU: 255 Category:

Product Description

Cat no 255

When bacteria are used to make medicinally useful proteins by transformation, the protein of interest must be separated from all of the other cellular proteins. In this experiment, the unique fluorescent properties of GFP and BFP will be used as an assay during their purification from an E. coli extract. The column fractions containing GFP or BFP will be identified by fluorescence and then purified. As an optional activity, purified protein fractions can be separated by SDS polyacrylamide gel electrophoresis (SDS-PAGE) to estimate the purity and size of the GFP and BFP proteins.

Kit includes: instructions, columns and matrix, GFP and BFP extracts, buffer, protein gel reagents for optional activity.

All you need: waterbath, long wave UV lamp (Cat no 969 recommended), ring stand & clamps, automatic micropipette, vertical gel electrophoresis apparatus, power supply, polyacrylamide gels (12%).

969_800

Cat no 969 UV lamp recommended