Blue/White Cloning of a DNA Fragment & Assay of ß-galactosidase

300 web

Blue/White Cloning of a DNA Fragment & Assay of ß-galactosidase

£175.00 Ex VAT

For 5 student groups

Complete 3 modules in 3 hours and 15 minutes

SKU: 300 Category:

Product Description

Cat no 300

When DNA is subcloned in the pUC polylinker region, ß-galactosidase production is interrupted, resulting in the inability of cells to hydrolyse X-Gal. This results in the production of white colonies amongst a background of blue colonies. This experiment provides a DNA fragment together with a linear plasmid and T4 DNA Ligase. Following the ligation to synthesize the recombinant plasmid, competent E. coli cells are transformed and the number of recombinant antibiotic resistant white and blue colonies are counted. ß-galactosidase activity is assayed from blue and white bacterial cells.

Kit includes: instructions, linearized pUC plasmid & DNA fragment, T4 ligase, bacterial LyphoCells for transformation,
reconstitution buffer, X-Gal in solvent, IPTG, calcium chloride, antibiotic, ReadyPour Luria Broth Agar, Luria broth media for
recovery, growth media, assay components, plastic supplies.

All you need: incubation oven, waterbaths, automatic micropipette and tips, spectrophotometer, centrifuge, microcentrifuge.