Construction and Cloning of a DNA Recombinant

301 web

Construction and Cloning of a DNA Recombinant

£295.00 Ex VAT

For 5 student groups

Complete 5 modules in about 5 hours

SKU: 301 Category:

Product Description

Cat no 301

Cloning is frequently performed to study gene structure, function, and to enhance gene expression. This experiment is divided into five modules. Clones are constructed by ligation of a vector and a fragment insert. The constructs are then transformed into competent cells and the cells are grown and selected for resistance. Plasmid DNA is then isolated from the transformants, cleaved with restriction enzymes, and analysed by agarose gel electrophoresis. Recommended for college level courses.

Kit includes: instructions, enzymes, plasmid DNA, restriction enzyme dilution buffer, enzyme grade water, standard DNA fragments, restriction enzyme reaction buffer, gel loading solution, agarose powder, electrophoresis buffer, stains, calibrated pipette.

All you need: electrophoresis apparatus and power supply, adjustable micropipette with tips, balance, microwave or hot plate, waterbath, large weigh boats for staining, UV transilluminator, floating racks for microtubes, pipette pump or bulb, 5 or 10 ml pipettes, laboratory glassware, rulers, distilled water, ice.