Exploring Biotechnology with Green Fluorescent Protein (GFP)

303 web

Exploring Biotechnology with Green Fluorescent Protein (GFP)

£175.00 Ex VAT

For 6 student groups

Complete 4 modules in 3 hours and 15 minutes

SKU: 303 Category:

Product Description

Cat no 303

Four experimental modules are combined into one to provide a comprehensive biotechnology exploration focusing on the green fluorescent protein (GFP). Bacterial cells are transformed to express the green fluorescent protein (GFP). The transformed cells are then grown and the GFP is purified by column chromatography. Finally, the purity of the protein fractions are analysed by SDS polyacrylamide electrophoresis.

Kit includes: instructions, transformation cells, plasmid DNA for GFP, IPTG, ampicillin antibiotic, calcium chloride, ReadyPour luria broth agar, luria broth media for recovery, petri plates, pipettes, calibrated transfer pipettes, inoculating loops, microtubes with attached caps, toothpicks, dry matrix for columns, chromatography columns, green and blue fluorescent protein extracts, elution buffer, protein molecular weight standards, protein denaturation solution, glycerol solution, tris-glycine-SDS buffer, stains

All you need: incubation oven, waterbaths, automatic micropipette and tips, long wave UV light, ring stand and clamps, lab glassware, ice, vertical gel electrophoresis apparatus and power supply, 3 polyacrylamide Gels (12%), glacial acetic acid, methanol.