Fermentation and Bioprocessing of GFP

305 web

Fermentation and Bioprocessing of GFP

£165.00 Ex VAT

For 10 student groups. Complete in 5-6 hours

SKU: 305 Category:

Product Description

Cat no 305

The Green Fluorescent Protein (GFP) has existed in the jellyfish, Aequorea victoria, for millions of years. Because of its bright fluorescence, GFP has become widely used in research as a reporter molecule. Today, the GFP protein is often fused to other proteins to study various biochemical processes. In this kit, students will express GFP protein using Escherichia coli in a small-scale fermentor. The conditions that are controlled during the fermentation are aeration, temperature, and IPTG concentrations. Bacterial growth and weight of GFP bacteria are monitored throughout the fermentation.

Kit includes: instructions, BactoBeads with GFP plasmid, LB growth media, growth additive, ampicillin, IPTG, GFP extraction buffer, microcentrifuge tubes, syringe, 15 ml centrifuge tubes, transfer pipets, loops

All you need: Air pump, heater, pH probe, temperature probe, fermentor vessel, autoclave, ice, shaker, ethanol, DH2O, centrifuge, colorimeter, balance